Research Paper Volume 13, Issue 11 pp 15013—15031

ERp44/CG9911 promotes fat storage in Drosophila adipocytes by regulating ER Ca2+ homeostasis

Characterization of CG9911 mutants. (A) Schematic presentation of CG9911 mutant mediated by CRISPR/Cas9. The scissors indicate where the CRISPR/Cas9 cleaves at the CG9911 locus. Red line indicates 5 bp deletion on genomic region. Two pairs of identification primers are marked (f1, r1) and (f2, r2) and located by opposite arrows. (B) The extracted genomic DNA of homozygous lines (f18, f19, f20, f29) is used as single fly PCR templates. The different molecular weight compared to w1118 indicates successful deletion. (C) Sequencing results show that 5 bp DNA are deleted before ‘NGG’.

Figure 1. Characterization of CG9911 mutants. (A) Schematic presentation of CG9911 mutant mediated by CRISPR/Cas9. The scissors indicate where the CRISPR/Cas9 cleaves at the CG9911 locus. Red line indicates 5 bp deletion on genomic region. Two pairs of identification primers are marked (f1, r1) and (f2, r2) and located by opposite arrows. (B) The extracted genomic DNA of homozygous lines (f18, f19, f20, f29) is used as single fly PCR templates. The different molecular weight compared to w1118 indicates successful deletion. (C) Sequencing results show that 5 bp DNA are deleted before ‘NGG’.