Research Paper Volume 13, Issue 11 pp 15013—15031

ERp44/CG9911 promotes fat storage in Drosophila adipocytes by regulating ER Ca2+ homeostasis

CG9911 mutant causes ER stress. (A) Survival curves of w1118 and CG9911f20 treated with tunicamycin. Log-rank (Mantel-Cox) test indicates p B) Real-time PCR detecting mRNA level of BiP, sXbp1 and Xbp1 in w1118 and CG9911f20 flies. Relative mRNA level was normalized to w1118. All gene detection was subject to three independent replicates. Data are presented as the means ± s.e.m; *pC) Lipid droplets staining of fat bodies in adult flies with and without TM treated. BODIPY was used to stain lipid droplets (green), and nuclei is stained by DAPI. (D) The proportion of stained LD area in the micrograph. Data are presented as the means ± s.e.m; ** pE) TAG level of adult flies treated with and without TM in three independent trials. Data were presented as the means ± s.e.m. * p F) Three independent real-time PCR detection of lipogenesis and lipolysis related genes in TM-induced flies at 48h. Data are presented as the means ± s.e.m; * p

Figure 3. CG9911 mutant causes ER stress. (A) Survival curves of w1118 and CG9911f20 treated with tunicamycin. Log-rank (Mantel-Cox) test indicates p < 0.0001. (B) Real-time PCR detecting mRNA level of BiP, sXbp1 and Xbp1 in w1118 and CG9911f20 flies. Relative mRNA level was normalized to w1118. All gene detection was subject to three independent replicates. Data are presented as the means ± s.e.m; *p<0.05, ** p<0.01. (C) Lipid droplets staining of fat bodies in adult flies with and without TM treated. BODIPY was used to stain lipid droplets (green), and nuclei is stained by DAPI. (D) The proportion of stained LD area in the micrograph. Data are presented as the means ± s.e.m; ** p<0.01. (E) TAG level of adult flies treated with and without TM in three independent trials. Data were presented as the means ± s.e.m. * p < 0.05 and **p < 0.01. (F) Three independent real-time PCR detection of lipogenesis and lipolysis related genes in TM-induced flies at 48h. Data are presented as the means ± s.e.m; * p < 0.05.