Research Paper Volume 13, Issue 11 pp 15013—15031

ERp44/CG9911 promotes fat storage in Drosophila adipocytes by regulating ER Ca2+ homeostasis

The phenotype of CG9911 mutants is rescued by ER Ca2+ reduction. (A) Knockdown of IP3R rescued lipid storage phenotype of CG9911 mutants. Lipid droplets were stained by BODIPY and nuclei were stained by DAPI. Scale bar =25 μm. (B) The percentage of stained LD area in the micrograph. Over 10 micrographs in each group were considered for data analysis. Data are presented as the means ± s.e.m; *pC) Relative glyceride levels were examined in adult fat bodies with different genotypes in three independent trials. Glyceride levels were normalized to protein content. Data are presented as the means ± s.e.m; *p

Figure 4. The phenotype of CG9911 mutants is rescued by ER Ca2+ reduction. (A) Knockdown of IP3R rescued lipid storage phenotype of CG9911 mutants. Lipid droplets were stained by BODIPY and nuclei were stained by DAPI. Scale bar =25 μm. (B) The percentage of stained LD area in the micrograph. Over 10 micrographs in each group were considered for data analysis. Data are presented as the means ± s.e.m; *p<0.05, ** p<0.01. (C) Relative glyceride levels were examined in adult fat bodies with different genotypes in three independent trials. Glyceride levels were normalized to protein content. Data are presented as the means ± s.e.m; *p < 0.05.