Research Paper Volume 13, Issue 11 pp 15044—15060

Integrated analysis of long non-coding RNAs (lncRNAs) and mRNA expression profiles identifies lncRNA PRKG1-AS1 playing important roles in skeletal muscle aging

The effect of knock-down of PRKG1-AS1 on muscle regulatory factors. (A) qRT-PCR analyzes gene expression of MyoD, MyoG, Myf2c and Myf5. Dexamethasone (15 mM) was added in human skeletal muscle myoblasts to establish atrophy cell model. Si-PRKG1-AS1 or siNC was transfected into human skeletal muscle myoblasts and incubated for 48 h. * P P P P P B) Protein expression of MyoD, MyoG, Myf2c and Myf5 detected by western blot. Dexamethasone (15 mM) was added in human skeletal muscle myoblasts to establish atrophy cell model. Si-PRKG1-AS1 or siNC was transfected into human skeletal muscle myoblasts and incubated for 48 h.

Figure 8. The effect of knock-down of PRKG1-AS1 on muscle regulatory factors. (A) qRT-PCR analyzes gene expression of MyoD, MyoG, Myf2c and Myf5. Dexamethasone (15 mM) was added in human skeletal muscle myoblasts to establish atrophy cell model. Si-PRKG1-AS1 or siNC was transfected into human skeletal muscle myoblasts and incubated for 48 h. * P < 0.05, ** P < 0.01 compared with control group; ## P < 0.01 compared with model group; & P < 0.05, && P < 0.01 compared with siNC group. (B) Protein expression of MyoD, MyoG, Myf2c and Myf5 detected by western blot. Dexamethasone (15 mM) was added in human skeletal muscle myoblasts to establish atrophy cell model. Si-PRKG1-AS1 or siNC was transfected into human skeletal muscle myoblasts and incubated for 48 h.