Research Paper Volume 13, Issue 10 pp 13474—13495

Ganoderma lucidum stimulates autophagy-dependent longevity pathways in Caenorhabditis elegans and human cells

G. lucidum inhibits the mTOR pathway in human cells. (A) Effects of G. lucidum (GL) on the mTOR pathway in human Huh7 hepatoma cells. Cells were treated with GL for 4 hrs, prior to Western blot analysis. Expression was normalized against GAPDH. (B) Protein intensity evaluated by densitometry. (C, D) Effects of GL on the mTOR pathway in human IMR-90 lung fibroblasts. Cells were processed as above for (C) Western blotting and (D) densitometry analysis. Statistical analysis was done with one-way analysis of variance (ANOVA). *p

Figure 3. G. lucidum inhibits the mTOR pathway in human cells. (A) Effects of G. lucidum (GL) on the mTOR pathway in human Huh7 hepatoma cells. Cells were treated with GL for 4 hrs, prior to Western blot analysis. Expression was normalized against GAPDH. (B) Protein intensity evaluated by densitometry. (C, D) Effects of GL on the mTOR pathway in human IMR-90 lung fibroblasts. Cells were processed as above for (C) Western blotting and (D) densitometry analysis. Statistical analysis was done with one-way analysis of variance (ANOVA). *p<0.05; **p<0.01; ***p<0.001.