Research Paper Volume 13, Issue 11 pp 15078—15099

Mechanistic insights into the anti-depressant effect of emodin: an integrated systems pharmacology study and experimental validation

Effects of EMO on PI3K-Akt signaling pathway associated proteins in the PFC. (A) A schematic illustration of the PI3K-AKT pathway (hsa04151). (B–E) Levels of PFC total GSK3β (t-GSK3β) and phosphorylated GSK3β (p-GSK3β, Ser9), total AKT (t-AKT) and phosphorylated AKT (p-AKT, Ser473), and total ERK (t-ERK) and phosphorylated ERK (p-ERK, Thr202/Tyr204) were measured by Western blotting and quantitatively analyzed (n=4). Data were expressed as the means ± SEM. ▲ p p p

Figure 9. Effects of EMO on PI3K-Akt signaling pathway associated proteins in the PFC. (A) A schematic illustration of the PI3K-AKT pathway (hsa04151). (BE) Levels of PFC total GSK3β (t-GSK3β) and phosphorylated GSK3β (p-GSK3β, Ser9), total AKT (t-AKT) and phosphorylated AKT (p-AKT, Ser473), and total ERK (t-ERK) and phosphorylated ERK (p-ERK, Thr202/Tyr204) were measured by Western blotting and quantitatively analyzed (n=4). Data were expressed as the means ± SEM. ▲ p < 0.05 CON+EMO vs CON+VEH. *** p < 0.001 DET+VEH vs CON+VEH. ### p < 0.001 DET+EMO vs DET+VEH.