Research Paper Volume 13, Issue 11 pp 15100—15113

Hsa_circ_0032131 knockdown inhibits osteoarthritis progression via the miR-502-5p/PRDX3 axis

In vitro model of OA was successfully established. (A) CHON-001 cells were treated with 10 ng/mL IL-1β for 48 h. Next, the expression of MMP-1, MMP-13, and Aggrecan in CHON-001 cells was detected by western blotting. (B) The relative expression of MMP-1 was quantified by normalizing it to that of β-actin. (C) The relative expression of MMP-13 was quantified by normalizing it to that of β-actin. (D) The relative expression of Aggrecan was quantified by normalizing it to that of β-actin. **p

Figure 1. In vitro model of OA was successfully established. (A) CHON-001 cells were treated with 10 ng/mL IL-1β for 48 h. Next, the expression of MMP-1, MMP-13, and Aggrecan in CHON-001 cells was detected by western blotting. (B) The relative expression of MMP-1 was quantified by normalizing it to that of β-actin. (C) The relative expression of MMP-13 was quantified by normalizing it to that of β-actin. (D) The relative expression of Aggrecan was quantified by normalizing it to that of β-actin. **p < 0.01 compared with the control.