Research Paper Volume 13, Issue 11 pp 15100—15113

Hsa_circ_0032131 knockdown inhibits osteoarthritis progression via the miR-502-5p/PRDX3 axis

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Figure 3.

IL-1β-induced CHON-001 growth inhibition was reversed by hsa_circ_0032131 knockdown. (A, B) Apoptosis of CHON-001 cells was measured by flow cytometry. (C) The expression of Aggrecan, MMP-13, Bax, and active caspase 3 in CHON-001 cells was detected by western blotting. (D) The relative expression of MMP-13 was quantified by normalizing it to that of β-actin. (E) The relative expression of Bax was quantified by normalizing it to that of β-actin. (F) The relative expression of active caspase 3 was quantified by normalizing it to that of β-actin. (G) The relative expression of Aggrecan was quantified by normalizing it to that of β-actin. **p < 0.01 compared with the control. ##p < 0.01 compared with IL-1β.