Figure 3. Oxidative stress induces DNA damage and apoptosis in GCs. (A) ROS of GCs of women with normal ovarian function and POF patients was assessed via DHE probes. Scale bar: 100μm. (B) ROS in the KGN, CTX-KGN-PBS and CTX-KGN-BMSCs groups were assessed using DHE probes. Scale bar: 100μm. (C) ROS in the ovaries of the WT, POF-PBS and POF-BMSCs groups were assessed using DCFH-DA probes. Scale bar: 100μm. (D) Cells were treated with 0 μM to 200 μM H2O2 for 1 hour. Total production of ROS was assessed using DHE probes. Scale bar: 100μm. (E) The DNA damage of cells treated with H2O2 was measured via comet assay. Scale bar: 100μm. Comparison of the tail of DNA between the groups. (F) Apoptosis of H2O2+KGN was assessed using Annexin V staining. Comparison of apoptotic rates between the groups.