Research Paper Advance Articles

TRDMT1 participates in the DNA damage repair of granulosa cells in premature ovarian failure

TRDMT1 mediates GCs oxidative DNA damage repair. (A) The DNA damage of H2O2+KGN+PBS and H2O2+KGN+NAC transfected with GFP-NC, GFP-TRDMT1, Sh-RNA and Sh-TRDMT1 was measured via comet assay. Scale bar: 100μm. Comparison of the tail of DNA between the groups. (B) H2O2+KGN+PBS transfected with GFP-NC, GFP-TRDMT1, Sh-RNA and Sh-TRDMT1 were harvested at the indicated time points for γ-H2AX staining. Positive cells in each cell and the frequency of cells with foci were counted. Scale bar: 10μm. (C) H2O2+KGN+NAC transfected with GFP-NC, GFP-TRDMT1, Sh-RNA and Sh-TRDMT1 were harvested at the indicated time points for γ-H2AX staining. Positive cells in each group and the frequency of cells with foci were counted. Scale bar: 10μm.*P

Figure 4. TRDMT1 mediates GCs oxidative DNA damage repair. (A) The DNA damage of H2O2+KGN+PBS and H2O2+KGN+NAC transfected with GFP-NC, GFP-TRDMT1, Sh-RNA and Sh-TRDMT1 was measured via comet assay. Scale bar: 100μm. Comparison of the tail of DNA between the groups. (B) H2O2+KGN+PBS transfected with GFP-NC, GFP-TRDMT1, Sh-RNA and Sh-TRDMT1 were harvested at the indicated time points for γ-H2AX staining. Positive cells in each cell and the frequency of cells with foci were counted. Scale bar: 10μm. (C) H2O2+KGN+NAC transfected with GFP-NC, GFP-TRDMT1, Sh-RNA and Sh-TRDMT1 were harvested at the indicated time points for γ-H2AX staining. Positive cells in each group and the frequency of cells with foci were counted. Scale bar: 10μm.*P<0.05, **p<0.01, and ***p<0.001. Statistical significance was determined using two-tailed t-tests for two groups and ANOVA for multiple comparisons. All values are the means ± SD.