Research Paper Volume 13, Issue 10 pp 13421—13429

Systemic NF-κB-mediated inflammation promotes an aging phenotype in skeletal stem/progenitor cells

Increased NF-κB-mediated inflammation leads to impaired differentiation potential. (A, B) Osteogenic potential was assessed by Alizarin red staining (A), which demonstrated significantly reduced mineralization in Nfkb1-/-WT and WTNfkb1-/- samples compared to control (B) (n=3, **** P ). (C, D) Significantly increased lipid accumulation was observed in Nfkb1-/-WT and WTNfkb1-/- samples as revealed by Oil Red O staining (C) and quantification (D) (n=3, *** P ≤ 0.001, **** P ≤ 0.0001).

Figure 3. Increased NF-κB-mediated inflammation leads to impaired differentiation potential. (A, B) Osteogenic potential was assessed by Alizarin red staining (A), which demonstrated significantly reduced mineralization in Nfkb1-/-WT and WTNfkb1-/- samples compared to control (B) (n=3, **** P < 0.0001). (C, D) Significantly increased lipid accumulation was observed in Nfkb1-/-WT and WTNfkb1-/- samples as revealed by Oil Red O staining (C) and quantification (D) (n=3, *** P0.001, **** P0.0001).