Research Paper Volume 13, Issue 11 pp 15285—15306

Mesenchymal stem cells-derived extracellular vesicles ameliorate Alzheimer’s disease in rat models via the microRNA-29c-3p/BACE1 axis and the Wnt/β-catenin pathway

BM-MSC-EVs have therapeutic effects on AD hippocampal neurons. The AD neuron model was established by Aβ1-42 induction, and then AD neurons were treated with BM-MSC-EVs or equal volume of BM-MSC conditioned medium after GW4869 treatment. (A) Immunofluorescence assay was used to detect Aβ content in hippocampal neurons. (B) ELISA was used to detect level of Aβ1-42; (C) MTT assay was used to detect the viability of AD hippocampal neurons; (D) Flow cytometry was used to detect the apoptosis rate of AD hippocampal neurons. The experiment was repeated three times, and the data was expressed as mean ± standard deviation. Data were analyzed using one-way ANOVA followed by Tukey’s multiple comparisons test. **p p

Figure 2. BM-MSC-EVs have therapeutic effects on AD hippocampal neurons. The AD neuron model was established by Aβ1-42 induction, and then AD neurons were treated with BM-MSC-EVs or equal volume of BM-MSC conditioned medium after GW4869 treatment. (A) Immunofluorescence assay was used to detect Aβ content in hippocampal neurons. (B) ELISA was used to detect level of Aβ1-42; (C) MTT assay was used to detect the viability of AD hippocampal neurons; (D) Flow cytometry was used to detect the apoptosis rate of AD hippocampal neurons. The experiment was repeated three times, and the data was expressed as mean ± standard deviation. Data were analyzed using one-way ANOVA followed by Tukey’s multiple comparisons test. **p < 0.01, ***p < 0.001.