Research Paper Volume 13, Issue 11 pp 15353—15365

Alisol A 24-acetate protects against brain microvascular endothelial cells injury through inhibiting miR-92a-3p/tight junctions axis

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Figure 2. Effects of Alisol A 24-acetate on the morphology and viability of bEnd.3 cells after OGD injury. (A) Cells were observed under an inverted microscope after oxygen-glucose deprivation (OGD) (scale bar 100 μm). (B) Cell viability was calculated by the CCK-8 assay. (C) Secreted LDH levels in cell culture supernatant were determined by the LDH kit (OGD-L: 1.9 umol/L Alisol A 24-acetate; OGD-M: 19 umol/L Alisol A 24-acetate; OGD-H: 38 umol/L Alisol A 24-acetate). Results are described as means ± SD (n = 6) (**P < 0.01 vs. control group, ##P < 0.01 vs. OGD group).