Research Paper Volume 13, Issue 11 pp 15353—15365

Alisol A 24-acetate protects against brain microvascular endothelial cells injury through inhibiting miR-92a-3p/tight junctions axis

Effects of miR-92a-3p transfection on the expression of TJ proteins. (A–C) Western blot analysis was performed to determine the expression of ZO-1, claudin-5, and occludin. The amount of TJ protein was quantified and normalized to that of β-actin (miNC: mimics negative control; inNC: inhibitor negative control; mi92: miR-92a-3p mimics; mi92+M: miR-92a-3p mimics +19 umol/L Alisol A 24-acetate; in92: miR-92a-3p inhibitor). Results are described as means ± SD (n = 3) (**P #P ##P @@P

Figure 6. Effects of miR-92a-3p transfection on the expression of TJ proteins. (AC) Western blot analysis was performed to determine the expression of ZO-1, claudin-5, and occludin. The amount of TJ protein was quantified and normalized to that of β-actin (miNC: mimics negative control; inNC: inhibitor negative control; mi92: miR-92a-3p mimics; mi92+M: miR-92a-3p mimics +19 umol/L Alisol A 24-acetate; in92: miR-92a-3p inhibitor). Results are described as means ± SD (n = 3) (**P < 0.01 vs. control group; #P < 0.05, ##P < 0.01 vs. OGD group; @@P < 0.01 vs. mi92a group).