Research Paper Volume 13, Issue 11 pp 15384—15399

CXCR5 induces perineural invasion of salivary adenoid cystic carcinoma by inhibiting microRNA-187

CXCR5 promotes the expression of Schwann cell markers by inhibiting miR-187. (A) An RNA-FISH assay was conducted to determine the level of miR-187 in normal salivary gland (NSG), SACC without PNI (No-PNI), far away from nerve of SACC with PNI (Far-nerve), nerve invasion front of SACC with PNI (Nerve-front). ‘N’ represented nerve, (Bar: 50 μm). (B) The binding sites on S100A4 3′UTR for miR-187 and the mutant sites. (C) Luciferase reporter analysis was carried out to determine the interaction of miR-187 with S100A4. (D) qRT-PCR analysis showed that reduced expression of Schwann cell hallmarks mediated by CXCR5 silence could be partially reversed by miR-187 inhibitor. (E) Inhibitory migratory, invasive and PNI ability in SACC-LM cells mediated by CXCR5 silence could be reversed by S100A4 overexpression. (F) Repressive migratory, invasive and PNI ability in SACC-LM cells induced miR-187 overexpression was alleviated by S100A4 overexpression, (*p **p

Figure 4. CXCR5 promotes the expression of Schwann cell markers by inhibiting miR-187. (A) An RNA-FISH assay was conducted to determine the level of miR-187 in normal salivary gland (NSG), SACC without PNI (No-PNI), far away from nerve of SACC with PNI (Far-nerve), nerve invasion front of SACC with PNI (Nerve-front). ‘N’ represented nerve, (Bar: 50 μm). (B) The binding sites on S100A4 3′UTR for miR-187 and the mutant sites. (C) Luciferase reporter analysis was carried out to determine the interaction of miR-187 with S100A4. (D) qRT-PCR analysis showed that reduced expression of Schwann cell hallmarks mediated by CXCR5 silence could be partially reversed by miR-187 inhibitor. (E) Inhibitory migratory, invasive and PNI ability in SACC-LM cells mediated by CXCR5 silence could be reversed by S100A4 overexpression. (F) Repressive migratory, invasive and PNI ability in SACC-LM cells induced miR-187 overexpression was alleviated by S100A4 overexpression, (*p < 0.05, **p < 0.01).