Research Paper Volume 13, Issue 11 pp 15384—15399

CXCR5 induces perineural invasion of salivary adenoid cystic carcinoma by inhibiting microRNA-187

Inhibition of CXCR5 suppresses PNI in SACC xenograft model. (A) Representative images on hindlimb function of the mouse and HE staining were presented. (B) qRT-PCR analysis showed that anti-CXCR5 antibody group mice showed reduced expression of S100A4, p75NTR and GFAP compared with saline group mice. (C) Immunohistochemical staining showed that anti-CXCR5 antibody group mice showed reduced expression of S100A4, p75NTR and GFAP compared with saline group mice. The expression of S100A4, p75NTR, GFAP was higher at nerve invasion frontier than that in tumors far away from nerve in PNI groups, ‘N’ represented nerve, (Bar: 50 μm) (**p ***p

Figure 5. Inhibition of CXCR5 suppresses PNI in SACC xenograft model. (A) Representative images on hindlimb function of the mouse and HE staining were presented. (B) qRT-PCR analysis showed that anti-CXCR5 antibody group mice showed reduced expression of S100A4, p75NTR and GFAP compared with saline group mice. (C) Immunohistochemical staining showed that anti-CXCR5 antibody group mice showed reduced expression of S100A4, p75NTR and GFAP compared with saline group mice. The expression of S100A4, p75NTR, GFAP was higher at nerve invasion frontier than that in tumors far away from nerve in PNI groups, ‘N’ represented nerve, (Bar: 50 μm) (**p < 0.01, ***p < 0.001).