Figure 2. Age-induced changes in NF-κB activity and mRNA levels of SASP factors in the brainstem. (A) NF-κB DNA-binding capacity measured by ELISA in nuclear protein extracted from the brainstem of young and aged animals. (B) Gene expression levels of SASP factors in the brainstem measured by real-time PCR analysis. Data are expressed as mean±SE, n=4-5/group. *denotes a significant difference (p < 0.05) from young animals.