Figure 3. Aging induces glial senescence in the brainstem. (A) Purity of glial cell-enriched fraction assessed by real-time PCR analysis. (B–H) Gene expression analysis of senescence markers in the glial cell-enriched fraction from young and aged brainstem. Data are expressed as mean±SE, n=4-5/group. *denotes a significant difference (p < 0.05) from young animals.