Research Paper Volume 13, Issue 11 pp 15523—15537

S100A8 and S100A9, both transcriptionally regulated by PU.1, promote epithelial-mesenchymal transformation (EMT) and invasive growth of dermal keratinocytes during scar formation post burn

S100A8 and S100A9 were upregulated in post burn skin and thermal-stimulated keratinocytes. (A) Representative blots for S100A8 and S100A9 in burned and matched normal skin tissues from burn patients. Comparison of (B) S100A8 and (C) S100A9 in burned and matched normal skin tissues of 38 patients. (D) Expression of S100A8 and S100A9 and (F) EMT marker proteins in thermal-stimulated keratinocytes was detected with Western blotting at different time points. (E) Double immunofluorescence assay for cellular localization of S100A8 and S100A9 and (G) immunofluorescence assay for nuclear translocation of Snail protein in thermal-stimulated keratinocytes at different time points. ***P

Figure 1. S100A8 and S100A9 were upregulated in post burn skin and thermal-stimulated keratinocytes. (A) Representative blots for S100A8 and S100A9 in burned and matched normal skin tissues from burn patients. Comparison of (B) S100A8 and (C) S100A9 in burned and matched normal skin tissues of 38 patients. (D) Expression of S100A8 and S100A9 and (F) EMT marker proteins in thermal-stimulated keratinocytes was detected with Western blotting at different time points. (E) Double immunofluorescence assay for cellular localization of S100A8 and S100A9 and (G) immunofluorescence assay for nuclear translocation of Snail protein in thermal-stimulated keratinocytes at different time points. ***P < 0.001.