Research Paper Volume 13, Issue 11 pp 15523—15537
S100A8 and S100A9, both transcriptionally regulated by PU.1, promote epithelial-mesenchymal transformation (EMT) and invasive growth of dermal keratinocytes during scar formation post burn
Figure 2. S100A8 and S100A9 promote cell proliferation, expression of EMT marker proteins, and cell invasion in primary keratinocytes. Human primary keratinocytes were treated with 1 μg of rS100A8, 1 μg of rS100A9, 0.5 μg of rS100A8 plus 0.5 μg of rS100A9, or 1 μg of rS100A8 plus 1 μg of rS100A9, heat-inactivated rS100A8/rS100A9 were applied as a negative control. After incubation for 48 h, (A) cell number, (B) expression levels of EMT marker proteins, (C) proportion of CD44+CD24- cells, and (D) cell invasion, were respectively detected with automatic cell counter, Western blotting, FACS, and Transwell migration assay. *P < 0.05 vs. control, #P < 0.05 vs 1 μg rS100A8, $P < 0.05 vs. 0.5 μg rS100A8 + 0.5 μg rS100A9.