Research Paper Volume 13, Issue 11 pp 15538—15547

miR-34a inhibits melanoma growth by targeting ZEB1

miR-34a inhibits proliferation and migration of melanoma cells. (A, B) Western blotting and real-time PCR analysis of miR-34a and ZEB1 in cells overexpressing miR-34a;** PC, D) Western blotting and real-time PCR analysis of miR-34a and ZEB1 in cells with down-regulated miR-34a expression;** PE, F) MTT cell proliferation assay in A375 and A875 cells transfected with miR-34a mimic/inhibitor (mean ± SEM; ** PG–J) Transwell cell migration assay in A375 and A875 cells transfected with miR-34a mimic/inhibitor (mean ± SEM; ** P

Figure 2. miR-34a inhibits proliferation and migration of melanoma cells. (A, B) Western blotting and real-time PCR analysis of miR-34a and ZEB1 in cells overexpressing miR-34a;** P< 0.05 vs. control. (C, D) Western blotting and real-time PCR analysis of miR-34a and ZEB1 in cells with down-regulated miR-34a expression;** P< 0.05 vs. control. (E, F) MTT cell proliferation assay in A375 and A875 cells transfected with miR-34a mimic/inhibitor (mean ± SEM; ** P< 0.05 vs. control). (GJ) Transwell cell migration assay in A375 and A875 cells transfected with miR-34a mimic/inhibitor (mean ± SEM; ** P< 0.05 vs. control).