Figure 3. Effects of REST on the expression of synaptic genes. (A) REST was successfully knocked down in SH-SY5Y cells. n = 3. (B) REST knockdown increased GRIA1 and GRIN2A expression, while declined GRIN1 expression in SH-SY5Y cells. n = 3. (C) 10 μM Aβ increased the mRNA expression of GRIA1 and GRIN2A, and decreased GRIN1 mRNA expression. n = 3. (D) REST over-expression significantly increased the mRNA expression of GRIN1, which was decreased by 10 μM Aβ in SH-SY5Y cells. n = 3. Independent experiments were performed three times. Data are expressed as mean ± SEM. *p < 0.05, **p < 0.01 and ***p < 0.001 vs the corresponding control group.