Research Paper Advance Articles

Repressor element-1 silencing transcription factor regulates glutamate receptors and immediate early genes to affect synaptic plasticity

Effects of REST on the expression of synaptic genes. (A) REST was successfully knocked down in SH-SY5Y cells. n = 3. (B) REST knockdown increased GRIA1 and GRIN2A expression, while declined GRIN1 expression in SH-SY5Y cells. n = 3. (C) 10 μM Aβ increased the mRNA expression of GRIA1 and GRIN2A, and decreased GRIN1 mRNA expression. n = 3. (D) REST over-expression significantly increased the mRNA expression of GRIN1, which was decreased by 10 μM Aβ in SH-SY5Y cells. n = 3. Independent experiments were performed three times. Data are expressed as mean ± SEM. *p **p ***p

Figure 3. Effects of REST on the expression of synaptic genes. (A) REST was successfully knocked down in SH-SY5Y cells. n = 3. (B) REST knockdown increased GRIA1 and GRIN2A expression, while declined GRIN1 expression in SH-SY5Y cells. n = 3. (C) 10 μM Aβ increased the mRNA expression of GRIA1 and GRIN2A, and decreased GRIN1 mRNA expression. n = 3. (D) REST over-expression significantly increased the mRNA expression of GRIN1, which was decreased by 10 μM Aβ in SH-SY5Y cells. n = 3. Independent experiments were performed three times. Data are expressed as mean ± SEM. *p < 0.05, **p < 0.01 and ***p < 0.001 vs the corresponding control group.