Research Paper Volume 13, Issue 12 pp 16072—16087

MiR-513b-5p represses autophagy during the malignant progression of hepatocellular carcinoma by targeting PIK3R3

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Figure 6. PIK3R3 is involved in miR-513b-5p-inhibited autophagy liver cancer cells. (AF) The HepG2 and Huh-7 cells were treated with miR-513b-5p mimic and pcDNA3.1- PIK3R3. (A and B) The detection of LC3, beclin1, and p62 expression using Western blot analysis. (C and D) The analysis of cell proliferation using MTT assays. (E and F) The analysis of cell apoptosis using flow cytometry.