Research Paper Volume 13, Issue 12 pp 16072—16087

MiR-513b-5p represses autophagy during the malignant progression of hepatocellular carcinoma by targeting PIK3R3

Autophagy inhibitor 3-MA reverses miR-513b-5p-mediated liver cancer progression in vitro. (A–F) The HepG2 and Huh-7 cells were treated with miR-513b-5p mimic and 3-MA (5mM). (A and B) The analysis of cell proliferation using MTT assays. (C and D) The analysis of cell apoptosis using flow cytometry. (E and F) The analysis of cell migration/invasion using transwell assays.

Figure 7. Autophagy inhibitor 3-MA reverses miR-513b-5p-mediated liver cancer progression in vitro. (AF) The HepG2 and Huh-7 cells were treated with miR-513b-5p mimic and 3-MA (5mM). (A and B) The analysis of cell proliferation using MTT assays. (C and D) The analysis of cell apoptosis using flow cytometry. (E and F) The analysis of cell migration/invasion using transwell assays.