Figure 3. Levobupivacaine induces ferroptosis in NSCLC cells. (A and B) The A549 and A427 cells were co-treated with 5 mmol/L erastin or ferrostatin (1 mmol/L) and 2 mM levobupivacaine. The cell growth was analyzed by MTT assays. (C–G) The A549 and A427 cells were treated with levobupivacaine (2 mM) or equal volume saline. The erastin was used as a positive control. (C) The levels of ROS were measure by flow cytometry analysis in the cells. (D and E) The levels of iron and Fe2+ were analyzed by Iron Assay Kit. (F and G) The expression of GPX4, SLC7A11, and β-actin was measured by Western blot analysis in the cells. Data are presented as mean ± SD. Statistic significant differences were indicated: *P < 0.05, **P < 0.01.