Research Paper Volume 13, Issue 12 pp 16485—16499

Cell landscape atlas for patients with chronic thromboembolic pulmonary hypertension after pulmonary endarterectomy constructed using single-cell RNA sequencing

Characterization of fibroblasts/smooth muscle cells and endothelial cells in the pulmonary endarterectomized tissues of CTEPH patients. (A) UMAP plot of three subsets of fibroblasts/smooth muscle cells. (B) Violin plot illustrating fibroblast/smooth muscle cell/myofibroblast marker genes, including ACTA2, PDGFRB, COL1A1, COL3A1, S100A4, PDGFRA, DES, CNN1, and MYH11. (C) GO enrichment analysis of fibroblast/smooth muscle cell/myofibroblast markers. (D) Violin plot showing marker genes of endothelial cells, including END1, VWF, CD34, and CDH5. (E) GO enrichment analysis of endothelial cell marker genes. Abbreviations: CTEPH: chronic thromboembolic pulmonary hypertension; UMAP: Uniform Manifold Approximation and Projection; GO: Gene ontology.

Figure 3. Characterization of fibroblasts/smooth muscle cells and endothelial cells in the pulmonary endarterectomized tissues of CTEPH patients. (A) UMAP plot of three subsets of fibroblasts/smooth muscle cells. (B) Violin plot illustrating fibroblast/smooth muscle cell/myofibroblast marker genes, including ACTA2, PDGFRB, COL1A1, COL3A1, S100A4, PDGFRA, DES, CNN1, and MYH11. (C) GO enrichment analysis of fibroblast/smooth muscle cell/myofibroblast markers. (D) Violin plot showing marker genes of endothelial cells, including END1, VWF, CD34, and CDH5. (E) GO enrichment analysis of endothelial cell marker genes. Abbreviations: CTEPH: chronic thromboembolic pulmonary hypertension; UMAP: Uniform Manifold Approximation and Projection; GO: Gene ontology.