Figure 3. The effects of circ_0081001 on proliferation, migration, invasion, and apoptosis of OS cells. (A) qRT-PCR was used for detecting circ_0081001 expression in OS cell lines and HFOB 1.19 cells. (B) qRT-PCR was used for assaying the transfection efficiency of circ_0081001 overexpression plasmid and sh-circ_0081001 in OS cells. (C) CCK-8 method was employed for detecting the viability of 143B and MG63 cells. (D) EdU method was employed for examining the proliferation of 143B and MG63 cells. (E) Transwell assay was employed for examining the migration and invasion of 143B and MG63 cells. (F) The apoptosis of 143B and MG63 cells was evaluated by flow cytometry. ** and *** represent P<0.01 and P<0.001, respectively.