Research Paper Volume 13, Issue 15 pp 19272—19281

lncRNA ANRIL aggravates the chemoresistance of pancreatic cancer cells to gemcitabine by targeting inhibition of miR-181a and targeting HMGB1-induced autophagy

ANRIL increases resistance of pancreatic cancer cells to gemcitabine chemotherapy. (A) MTT assay showed that overexpression of ANRIL resisted the inhibition of pancreatic cancer cells by gemcitabine, but miR-181a mimics reversed the effect which caused by ANRIL. OD value reflected the relative proliferative activity of each group. (B) Colony formation assays suggested the effects of miR-181a, ANRIL and gemcitabine on the proliferation of PANC-1 cells and BxPC-3 cells. *P **P ***P

Figure 5. ANRIL increases resistance of pancreatic cancer cells to gemcitabine chemotherapy. (A) MTT assay showed that overexpression of ANRIL resisted the inhibition of pancreatic cancer cells by gemcitabine, but miR-181a mimics reversed the effect which caused by ANRIL. OD value reflected the relative proliferative activity of each group. (B) Colony formation assays suggested the effects of miR-181a, ANRIL and gemcitabine on the proliferation of PANC-1 cells and BxPC-3 cells. *P < 0.05, **P < 0.01, ***P < 0.001.