Research Paper Volume 13, Issue 15 pp 19282—19292

Evaluation of the cargo contents and potential role of extracellular vesicles in osteoporosis

Characterization and concentration analysis of extracellular vesicles. (A) Plasma-derived EVs isolated from four individuals with osteoporosis, four individuals without osteoporosis, one EV-depleted plasma sample were subjected to SDS-PAGE and probed for EV-enriched proteins, Annexin-V and TSG101. (B) Electron microscopy of EVs. Scale bar = 500 nm. (C) and (D) Nanoparticle tracking analysis (NTA). The area under the curve in (C) is shown in (D).

Figure 1. Characterization and concentration analysis of extracellular vesicles. (A) Plasma-derived EVs isolated from four individuals with osteoporosis, four individuals without osteoporosis, one EV-depleted plasma sample were subjected to SDS-PAGE and probed for EV-enriched proteins, Annexin-V and TSG101. (B) Electron microscopy of EVs. Scale bar = 500 nm. (C) and (D) Nanoparticle tracking analysis (NTA). The area under the curve in (C) is shown in (D).