Research Paper Volume 13, Issue 13 pp 17818—17829

Particulate matter exposure is highly correlated to pediatric asthma exacerbation

IL-33 antibody alleviated the aggravation of airway inflammation caused by PM exposure in the HDM-induced allergic mice. Male mice in the control and experimental groups were injected intraperitoneally with IL-33 antibody or control antibody, respectively (N = 8 for each group). Protocol for the IL-33 antibody or control antibody intervention (A). Total inflammatory cells, eosinophil proportion, and eosinophil number were analyzed (B–D). Protein levels of IL-33 (E) and IL-25 (F) in the lung tissues were measured using ELISA. Representative images of lung tissues stained with H&E (G) and PAS (I) were showed under the microscope (10 × 20 magnification). Inflammatory score (H) and mucus production (J) with semi-quantification (score: 0–4) were analyzed under the microscope (10 × 20 magnification). Data were presented as means ± SEM (*, P

Figure 5. IL-33 antibody alleviated the aggravation of airway inflammation caused by PM exposure in the HDM-induced allergic mice. Male mice in the control and experimental groups were injected intraperitoneally with IL-33 antibody or control antibody, respectively (N = 8 for each group). Protocol for the IL-33 antibody or control antibody intervention (A). Total inflammatory cells, eosinophil proportion, and eosinophil number were analyzed (BD). Protein levels of IL-33 (E) and IL-25 (F) in the lung tissues were measured using ELISA. Representative images of lung tissues stained with H&E (G) and PAS (I) were showed under the microscope (10 × 20 magnification). Inflammatory score (H) and mucus production (J) with semi-quantification (score: 0–4) were analyzed under the microscope (10 × 20 magnification). Data were presented as means ± SEM (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001).