Research Paper Volume 13, Issue 15 pp 19878—19893

CCL2 associated with CD38 expression during ex vivo expansion in human cord blood-derived hematopoietic stem cells

Identify CD34 and CD38 expression by flow cytometry after HSCs cultured for 7 days. (A) The isolated HSC (CD34+/CD38–) were examined by flow cytometry with SSC-conjugated anti-CD34 and FSC-conjugated anti-CD38. Non-stained HSCs are used to determine the background auto-fluorescence to set the negative population allowing cells stained with CD38 and CD34 to be visualized (right side). The percentage of surface marker-positive cells in the population is indicated. (B) Flow cytometry expression of CD34 and CD38 on hematopoietic stem cells. Comparison of mean fluorescence intensity (MFI) of CD34 and CD38 in serum-expanded (DAY7-FBS) vs. serum-free-expanded (DAY7-SF) HSCs.

Figure 2. Identify CD34 and CD38 expression by flow cytometry after HSCs cultured for 7 days. (A) The isolated HSC (CD34+/CD38) were examined by flow cytometry with SSC-conjugated anti-CD34 and FSC-conjugated anti-CD38. Non-stained HSCs are used to determine the background auto-fluorescence to set the negative population allowing cells stained with CD38 and CD34 to be visualized (right side). The percentage of surface marker-positive cells in the population is indicated. (B) Flow cytometry expression of CD34 and CD38 on hematopoietic stem cells. Comparison of mean fluorescence intensity (MFI) of CD34 and CD38 in serum-expanded (DAY7-FBS) vs. serum-free-expanded (DAY7-SF) HSCs.