Figure 1. The short form Cripto-1 (CR-1) mRNA is predominantly expressed in HCC cell lines and tissues. (A) Diagrammatic representation shows structure of the CR-1 gene and mRNAs (full-length and short-length forms). UN-D/UN-B: full-length CR-1 (FL-CR1) transcript specific primer set yields 432 bp PCR product; UN-A/UN-B: total CR-1 transcript specific primer set yields 305 bp PCR product. (B–C) RT-PCR analysis shows levels of FL-CR-1 transcript relative to total CR-1 (TCR) transcript levels in human HCC cell lines, QGY7701, Hep3B, HepG2, SK-Hep-1, SNU-387 and SNU-182. GAPDH was used as internal control. (D–G) RT-PCR analysis shows levels of FL-CR-1 transcript relative to total CR-1 transcript in human HCC (T/C) and their corresponding adjacent non-tumor liver tissues (N/B).