Figure 7. KAT5 induces circSMARCA5 production. (A) The KAT5 expression pattern in RWPE-1, DU145, LNCaP, and PC3 cells. (B) Detection of pulled-down circSMARCA5 by qRT-PCR. (C) qRT-PCR of KAT5, miR-181b-5p, TIMP3, and miR-17-3p in DU145 cells with and without KAT5 overexpression. (D) U145 cells were transfected with circSMARCA5-expressing vector and KAT5-specific siRNA, alone or in combination. Migration and invasion were determined by Transwell assays. *, **, *** represent p ≤ 0.05, p ≤ 0.01, p ≤ 0.001, respectively. Assays were performed at least three times.