Research Paper Volume 13, Issue 16 pp 20383—20394

Hsa_circ_0134111 promotes osteoarthritis progression by regulating miR-224-5p/CCL1 interaction

Hsa

Figure 1. Hsa_circ_0134111 knockdown prevents IL-1β-induced growth inhibition in chondrocytes. (A) Chondrocytes were treated with hsa_circ_0134111 siRNA-ctrl, hsa_circ_0134111 siRNA1, hsa_circ_0134111 siRNA2, or hsa_circ_0134111 siRNA3 and RT-qPCR was performed to measure knockdown efficiency. (B) Chondrocytes were treated with IL-1β and hsa_circ_0134111 siRNA1, alone or in combination, and RT-qPCR was performed to measure hsa_circ_0134111 levels in cells. (C) Results of the CCK-8 assay, used to detect cell viability. (D, E) EdU and DAPI staining was used to measure chondrocyte proliferation. **P<0.01 compared with control; ##P<0.01 compared with hsa_circ_0134111 siRNA1.