Figure 4. CCL1 is targeted and negatively regulated by miR-224-5p. (A) Diagram showing binding sites for miR-224-5p in the CCL1 mRNA and nucleotide sequence of the mutant CCL1 3’-UTR. (B) A dual-luciferase reporter assay was used to confirm the binding of CCL1 to miR-224-5p. (C) Chondrocytes were treated with miR-224-5p agomir-ctrl or miR-224-5p agomir and RT-qPCR was performed to measure CCL1 levels in cells. (D, E) Western blot was used to detect the level of CCL1. **P<0.01, compared with control.