Figure 3. Hsa_circ_0091994 knockdown promoted the apoptosis and suppressed the wound healing activity in AGS and HGC-27 cells. AGS and HGC-27 cells were treated with shRNA-1 or shRNA-ctrl for 48 hr. (A, B) Cell apoptosis rate was measured in each group. (C, D) Wound healing assay was employed to evaluate the effect of hsa_circ_0091994 knockdown on wound healing activity of AGS and HGC-27 cell. (E) The protein expression of E-cadherin and α-SMA was examined using western blot assay. β-actin was used as a loading control. (F, G) The expressions of E-cadherin and α-SMA were quantified respectively. The**P<0.01, compared with blank; n = 3.