Research Paper Volume 13, Issue 17 pp 21587—21598

FOXN3 inhibits cell proliferation and invasion via modulating the AKT/MDM2/p53 axis in human glioma

FOXN3 modulates the expression of malignant phenotype-associated proteins in glioma cells. (A) Protein expression levels of PCNA and CCND1 were examined by Western blotting after transfection with FOXN3 expression vectors in U87MG cells or transfection with shRNA#1 in SF126 cells. (B) Protein expression levels of cleaved caspase 3 and Bcl2 were detected via Western blotting after transfection with FOXN3 expression vectors in U87MG cells or transfection with shRNA#1 in SF126 cells. (C) Protein expression levels of Vimentin and MMP9 were determined using Western blotting after transfection with FOXN3 expression vectors in U87MG cells or transfection with shRNA#1 in SF126 cells. **P

Figure 3. FOXN3 modulates the expression of malignant phenotype-associated proteins in glioma cells. (A) Protein expression levels of PCNA and CCND1 were examined by Western blotting after transfection with FOXN3 expression vectors in U87MG cells or transfection with shRNA#1 in SF126 cells. (B) Protein expression levels of cleaved caspase 3 and Bcl2 were detected via Western blotting after transfection with FOXN3 expression vectors in U87MG cells or transfection with shRNA#1 in SF126 cells. (C) Protein expression levels of Vimentin and MMP9 were determined using Western blotting after transfection with FOXN3 expression vectors in U87MG cells or transfection with shRNA#1 in SF126 cells. **P < 0.01. FOXN3, forkhead box N3; shRNA#1, short hairpin RNA #1 specifically targeting FOXN3; Abbreviations: PCNA: proliferating cell nuclear antigen; CCND1: cyclin D1; Bcl2: B cell lymphoma; MMP9: matrix metalloproteinase 9.