Research Paper

Figure 6. FLL inhibits osteoclastogenesis and function by downregulation of Trap activity and levels of Trap5b and NFATc1. (A–B) The levels of Trap activity and Trap-5b proteins in plasma markedly decreased after treatment with FLL (n = 10). (C–D) Trap mRNA expression in the OVX group was significantly higher than in the sham group. Estrogen and FLL suppressed the increase in ACP5 and NFATc1 mRNA expression. (n = 3). (E) Monocytes from bone marrow were cultured with M-CSF (2.5 μg/mL) and FLL seven days before being subjected to CCK-8 assay. (F) The monocytes were treated with M-CSF (2.5 μg/mL) and RANKL (5 μg/mL) for 7 days and assessed by tartrate-resistant acid phosphatase (Tracp). FLL (1 μg/mL) significantly suppressed Tracp activity. (n = 6). (G–H) Monocytes were treated with M-CSF (2.5 μg/mL) and RANKL (5 μg/mL) for 7 days and stained for Tracp and F-actin rings. ^#P < 0.05 compared with sham; ^##P < 0.01 compared with sham; ^*P < 0.05 compared with OVX; ^**P < 0.01 compared with OVX (n = 3).