Figure 2. Ct-SLCO1B3 expression in CRC cell lines and select effective interfere sequence. (A, B) The mRNA (A) and protein (B) levels of Ct-SLCO1B3 in the NCM460 normal human colon epithelial cell line and the SW480, SW620, HT29, HCT116, and RKO human CRC cell lines by qRT-PCR and western blot analysis, respectively. n=3; *P < 0.05, **P < 0.01 vs. NCM460. (C, D) HCT116 and SW480 cells were transiently transfected with si1-Ct-SLCO1B3, si2-Ct-SLCO1B3, si3-Ct-SLCO1B3, or si-NC. (C) The Ct-SLCO1B3 mRNA levels by qRT-PCR. (D) The Ct-OATP1B3 protein levels by western blot analysis. n=3; *P < 0.05, **P < 0.01 vs. si-NC. NC=si-NC, Si1=si1-Ct-SLCO1B3, Si2=si2-Ct-SLCO1B3 and Si3=si3-Ct-SLCO1B3.