Research Paper Volume 13, Issue 18 pp 22164—22175

SLCO1B3 promotes colorectal cancer tumorigenesis and metastasis through STAT3

Ct-SLCO1B3 knockdown in HCT116 cells downregulates p-STAT3, MMP-2, and MMP-9. HCT116 cells were transiently transfected with si1-Ct-SLCO1B3, si2-Ct-SLCO1B3, or si-NC with or without IL-6 stimulation (50 ng/mL). The protein levels of Ct-OATP1B3, p-STAT3, total STAT3, MMP-2, and MMP-9 were determined by western blot analysis. (A) Gel image. (B) Quantified protein levels without IL-6 stimulation. n=3, **P si1-Ct-SLCO1B3, Si-2=si2-Ct-SLCO1B3.

Figure 6. Ct-SLCO1B3 knockdown in HCT116 cells downregulates p-STAT3, MMP-2, and MMP-9. HCT116 cells were transiently transfected with si1-Ct-SLCO1B3, si2-Ct-SLCO1B3, or si-NC with or without IL-6 stimulation (50 ng/mL). The protein levels of Ct-OATP1B3, p-STAT3, total STAT3, MMP-2, and MMP-9 were determined by western blot analysis. (A) Gel image. (B) Quantified protein levels without IL-6 stimulation. n=3, **P < 0.01 vs. si-NC. NC=si-NC, Si-1=si1-Ct-SLCO1B3, Si-2=si2-Ct-SLCO1B3.