Research Paper Volume 13, Issue 19 pp 22883—22897

Transcriptome study of oleanolic acid in the inhibition of breast tumor growth based on high-throughput sequencing

Effects of OA on the cell cycle and apoptosis in MCF-7 cells. Cells were treated with 40 μM OA for various hours (0 h, 4 h, 6 h and 8 h). (A) PI staining was conducted to observe the effect of OA on the cell cycle in vitro. Representative images were shown. (B) At least three independent experiments were performed, and the samples were prepared in triplicate. The data represent the mean ± SD. *P C) Annexin V FITC/PI double staining was conducted to observe the effect of OA on apoptosis in vitro. Representative images were shown. (D) At least three independent experiments were performed, and the samples were prepared in triplicate. The data represent the mean ± SD. *p **P

Figure 2. Effects of OA on the cell cycle and apoptosis in MCF-7 cells. Cells were treated with 40 μM OA for various hours (0 h, 4 h, 6 h and 8 h). (A) PI staining was conducted to observe the effect of OA on the cell cycle in vitro. Representative images were shown. (B) At least three independent experiments were performed, and the samples were prepared in triplicate. The data represent the mean ± SD. *P < 0.05, compared to control group. (C) Annexin V FITC/PI double staining was conducted to observe the effect of OA on apoptosis in vitro. Representative images were shown. (D) At least three independent experiments were performed, and the samples were prepared in triplicate. The data represent the mean ± SD. *p < 0.05, **P < 0.01, compared to control group.