Research Paper Volume 13, Issue 19 pp 23004—23019

lncRNA MEG3 aggravated neuropathic pain and astrocyte overaction through mediating miR-130a-5p/CXCL12/CXCR4 axis

MEG3 was overexpressed and miR-130a-5p was knocked down in CCI rats. (A, B) The expression of MEG3 in the L4-L6 dorsal spinal cord of CCI rats and astrocytes was assayed by RT-qPCR 0, 1, 3, 5, 7, and 14 days after CCI and 6 hours, 12 hours, 1 day, 3 days, 7 days, and 14 days after LPS induction of astrocytes, respectively. (C, D) Expression of miR-130a-5p in CCI rats and astrocytes was measured by RT-qPCR at 0, 1, 3, 5, 7, and 14 days after CCI and 6 hours, 12 hours, 1 day, 3 days, 7 days, and 14 days after LPS induction of astrocytes, respectively. (E, F) Expression of CXCL12/CXCR4 and its downstream Rac1 and NF-κB in the dorsal spinal cord of CCI rats and astrocytes at 0, 1, 3, 5, 7, and 14 days and LPS-induced astrocytes at 6 hours, 12 hours, 1 day, 3 days, 7 days, and 14 days, respectively, was measured using WB. Data were expressed as mean±SD. n=5. NSP>0.05, *PPP

Figure 1. MEG3 was overexpressed and miR-130a-5p was knocked down in CCI rats. (A, B) The expression of MEG3 in the L4-L6 dorsal spinal cord of CCI rats and astrocytes was assayed by RT-qPCR 0, 1, 3, 5, 7, and 14 days after CCI and 6 hours, 12 hours, 1 day, 3 days, 7 days, and 14 days after LPS induction of astrocytes, respectively. (C, D) Expression of miR-130a-5p in CCI rats and astrocytes was measured by RT-qPCR at 0, 1, 3, 5, 7, and 14 days after CCI and 6 hours, 12 hours, 1 day, 3 days, 7 days, and 14 days after LPS induction of astrocytes, respectively. (E, F) Expression of CXCL12/CXCR4 and its downstream Rac1 and NF-κB in the dorsal spinal cord of CCI rats and astrocytes at 0, 1, 3, 5, 7, and 14 days and LPS-induced astrocytes at 6 hours, 12 hours, 1 day, 3 days, 7 days, and 14 days, respectively, was measured using WB. Data were expressed as mean±SD. n=5. NSP>0.05, *P<0.05, **P<0.01, ***P<0.001 (vs. sham group).