Priority Research Paper Volume 13, Issue 19 pp 22629—22648

Green tea catechins EGCG and ECG enhance the fitness and lifespan of Caenorhabditis elegans by complex I inhibition

EGCG and ECG induce SOD and CTL activity and a shift in lipid metabolism in the long term. SOD (A) or CTL (B) activity after treatment with 0.1% DMSO, 2.5 μM EGCG or 2.5 μM ECG for 24 h or 7 days. The representative outcome of lifespan assay of sod-2 mutants treated with 0.1% DMSO or 2.5 μM EGCG. (C) The representative outcome of lifespan assay of ctl-2 mutants treated with 0.1% DMSO or 2.5 μM ECG. (D) Triglyceride content in N2 wild-type nematodes after treatment with 0.1% DMSO, 2.5 μM EGCG or 2.5 μM ECG for 5 days, normalized to protein content. (E) P-values are as indicated in the graphs. See Table 1 for corresponding detailed data and statistical analyses of lifespan assays.

Figure 5. EGCG and ECG induce SOD and CTL activity and a shift in lipid metabolism in the long term. SOD (A) or CTL (B) activity after treatment with 0.1% DMSO, 2.5 μM EGCG or 2.5 μM ECG for 24 h or 7 days. The representative outcome of lifespan assay of sod-2 mutants treated with 0.1% DMSO or 2.5 μM EGCG. (C) The representative outcome of lifespan assay of ctl-2 mutants treated with 0.1% DMSO or 2.5 μM ECG. (D) Triglyceride content in N2 wild-type nematodes after treatment with 0.1% DMSO, 2.5 μM EGCG or 2.5 μM ECG for 5 days, normalized to protein content. (E) P-values are as indicated in the graphs. See Table 1 for corresponding detailed data and statistical analyses of lifespan assays.