Research Paper Volume 13, Issue 19 pp 23108—23118

METTL3 regulates hippocampal gene transcription via N6-methyladenosine methylation in sevoflurane-induced postoperative cognitive dysfunction mouse

The weakened binding affinity of METTL3 in POCD. The protein levels of METTL3, METTL14, WTAP, ALKBH5, FTO, YTHDF1, IGF2BP1 and HNRNPA2B1 in the hippocampuses of POCD mice. (A) The binding affinity of METTL3 on BDNF, SOX2, and SYN1, (B) CCND2,STAR, and TIGAR, (C), as well as BACE1 and IL17A (D) in hippocampal neurons of POCD mice shown by RIP-qPCR. The binding affinity of FTO, (E) ALKBH5 (F) and IGF2BP1 (G) on BDNF, SOX2, and SYN1 in hippocampal neurons of POCD mice shown by RIP-qPCR. Data are presented as the mean ± standard error of the mean of three individual experiments; p

Figure 3. The weakened binding affinity of METTL3 in POCD. The protein levels of METTL3, METTL14, WTAP, ALKBH5, FTO, YTHDF1, IGF2BP1 and HNRNPA2B1 in the hippocampuses of POCD mice. (A) The binding affinity of METTL3 on BDNF, SOX2, and SYN1, (B) CCND2,STAR, and TIGAR, (C), as well as BACE1 and IL17A (D) in hippocampal neurons of POCD mice shown by RIP-qPCR. The binding affinity of FTO, (E) ALKBH5 (F) and IGF2BP1 (G) on BDNF, SOX2, and SYN1 in hippocampal neurons of POCD mice shown by RIP-qPCR. Data are presented as the mean ± standard error of the mean of three individual experiments; p < 0.05 vs. control group.