Research Paper Volume 13, Issue 19 pp 23169—23181

LncRNA-SNHG1 promotes macrophage M2-like polarization and contributes to breast cancer growth and metastasis

Silencing of lncRNA-SNHG1 attenuated the pro-angiogenesis effect of M2 macrophage. MCF-7 cells were cultured using condition medium from RAW264.7 cells or BMDMs for 24 hours. (A–D) Cell migration of MCF-7 cells cultured with RAW264.7 cells condition medium (A, C) or BMDMs (B, D) were assessed by Transwell assay. one-way ANOVA was used for the statistical analysis. n=5 independent cell cultures. The bar indicates the SD values. **PE–H) Tube formation of HUVEC cells was counted in the presence of the condition medium from RAW264.7 cells (E, G) or BMDMs (F, H). one-way ANOVA was used for the statistical analysis. n=5 independent cell cultures. The bar indicates the SD values. **P

Figure 3. Silencing of lncRNA-SNHG1 attenuated the pro-angiogenesis effect of M2 macrophage. MCF-7 cells were cultured using condition medium from RAW264.7 cells or BMDMs for 24 hours. (AD) Cell migration of MCF-7 cells cultured with RAW264.7 cells condition medium (A, C) or BMDMs (B, D) were assessed by Transwell assay. one-way ANOVA was used for the statistical analysis. n=5 independent cell cultures. The bar indicates the SD values. **P<0.01. (EH) Tube formation of HUVEC cells was counted in the presence of the condition medium from RAW264.7 cells (E, G) or BMDMs (F, H). one-way ANOVA was used for the statistical analysis. n=5 independent cell cultures. The bar indicates the SD values. **P<0.01.