Research Paper Volume 13, Issue 19 pp 23193—23209

Network pharmacology for systematic understanding of Schisandrin B reduces the epithelial cells injury of colitis through regulating pyroptosis by AMPK/Nrf2/NLRP3 inflammasome

The inhibition of AMPK reduced the anti-inflammation effects of Schisandrin B on NLRP3 inflammasome. (A–D) p-AMPK, Nrf2, NLRP3 and Caspase-1 protein expressions in mouse colon tissue; (E–H) p-AMPK, Nrf2, NLRP3 and Caspase-1 protein expressions in intestinal epithelial cells induced by LPS + ATP. ##P$$P$$P

Figure 6. The inhibition of AMPK reduced the anti-inflammation effects of Schisandrin B on NLRP3 inflammasome. (AD) p-AMPK, Nrf2, NLRP3 and Caspase-1 protein expressions in mouse colon tissue; (EH) p-AMPK, Nrf2, NLRP3 and Caspase-1 protein expressions in intestinal epithelial cells induced by LPS + ATP. ##P<0.01 vs control group; **P<0.01 vs DSS- induced colitis group; $$P<0.01 vs Colitis+SCH group. Control: blank control group; Colitis: DSS- induced colitis group; Colitis+SCH: DSS- induced colitis mice with Schisandrin; Colitis+SCH+ AMPK i: DSS- induced colitis mice with Schisandrin and AMPK inhibitor. ##P<0.01 vs MDSO group; **P<0.01 vs LPS+ATP induced intestinal epithelial cells group; $$P<0.01 vs LPS+ATP+SCH group. MDSO: blank control group; LPS+ATP: intestinal epithelial cells with LPS+ATP group; LPS+ATP +SCH: intestinal epithelial cells induced by LPS+ATP with Schisandrin; LPS+ATP+SCH+AMPK i: intestinal epithelial cells induced by LPS+ATP, Schisandrin and AMPK inhibitor. SCH, Schisandrin B. Data were expressed as mean ± SEM.