Research Paper Volume 13, Issue 19 pp 23193—23209

Network pharmacology for systematic understanding of Schisandrin B reduces the epithelial cells injury of colitis through regulating pyroptosis by AMPK/Nrf2/NLRP3 inflammasome

Schisandrin B reduced epithelial cells injury of colitis through regulating pyroptosis. (A) cell proliferation, (B) LDH activity, (C) PI staining, (D) cell apoptosis rate in epithelial cells induced by LPS + ATP; (E) GSDMD protein expression in mouse colon tissue; (F) GSDMD protein expression in epithelial cells induced by LPS + ATP; (G) GSDMD protein expression in mouse colon tissue by Schisandrin B and AMPK inhibitor; (H) GSDMD protein expression in epithelial cells induced by LPS + ATP, Schisandrin B and AMPK inhibitor. ##P$$P$$P

Figure 7. Schisandrin B reduced epithelial cells injury of colitis through regulating pyroptosis. (A) cell proliferation, (B) LDH activity, (C) PI staining, (D) cell apoptosis rate in epithelial cells induced by LPS + ATP; (E) GSDMD protein expression in mouse colon tissue; (F) GSDMD protein expression in epithelial cells induced by LPS + ATP; (G) GSDMD protein expression in mouse colon tissue by Schisandrin B and AMPK inhibitor; (H) GSDMD protein expression in epithelial cells induced by LPS + ATP, Schisandrin B and AMPK inhibitor. ##P<0.01 vs control group; **P<0.01 vs DSS- induced colitis group; $$P<0.01 vs Colitis+SCH group. Control: blank control group; Colitis: DSS- induced colitis group; Colitis+SCH: DSS- induced colitis mice with Schisandrin; Colitis+SCH+ AMPK i: DSS- induced colitis mice with Schisandrin and AMPK inhibitor. ##P<0.01 vs MDSO group; **P<0.01 vs LPS+ATP induced intestinal epithelial cells group; $$P<0.01 vs LPS+ATP+SCH group. MDSO: blank control group; LPS+ATP: intestinal epithelial cells with LPS+ATP group; LPS+ATP +SCH: intestinal epithelial cells induced by LPS+ATP with Schisandrin; LPS+ATP+SCH+AMPK i: intestinal epithelial cells induced by LPS+ATP, Schisandrin and AMPK inhibitor. SCH, Schisandrin B. Data were expressed as mean ± SEM.