Figure 2. Silencing piR-36741 hindered osteogenic differentiation of BMSCs. BMSCs were infected with Lv-sh-NC and Lv-sh-piR-36741, and then cultured in osteogenic differentiation medium for 14 days. (A–C) The expression of piR-36741, and the mRNA and protein levels of METTL3, RUNX2, COL1A1, OCN and OPN were measured on day 14. (D) Images of ALP staining on day 14 (100×). (E) ALP activity was determined on day 14. (F) Images of ARS staining (100×). (G) Quantitative analysis of ARS accumulation on day 14. N = 5 in each group. *P < 0.05, **P < 0.01. Each test was independently repeated at least three times.