Research Paper Volume 13, Issue 19 pp 23376—23392

Potentiated lung adenocarcinoma (LUAD) cell growth, migration and invasion by lncRNA DARS-AS1 via miR-188-5p/ KLF12 axis

Knockdown of DARS-AS1 resulted in alleviated LUAD progression. (A) The DARS-AS1 expression level was significantly decreased in A549 cells compared to the si-NC group after transfection with si-DARS-AS1 (p B) CCK-8 (p C) BrdU (p D) colony formation (p E) An increased apoptosis rate was observed in the si-DARS-AS1 group compared to the si-NC group (upper right quadrant + lower right quadrant) (p F) migrating (p G) invading (p

Figure 2. Knockdown of DARS-AS1 resulted in alleviated LUAD progression. (A) The DARS-AS1 expression level was significantly decreased in A549 cells compared to the si-NC group after transfection with si-DARS-AS1 (p < 0.05). DARS-AS1 knockdown resulted in a lower proliferation rate of LUAD cells according to the (B) CCK-8 (p < 0.05), (C) BrdU (p < 0.01), and (D) colony formation (p < 0.01) assays compared to the si-NC group. (E) An increased apoptosis rate was observed in the si-DARS-AS1 group compared to the si-NC group (upper right quadrant + lower right quadrant) (p < 0.01). A549 cells transfected with si-DARS-AS1 exhibited less (F) migrating (p < 0.05) and (G) invading (p < 0.01) cells compared to the si-NC group.