Research Paper Volume 13, Issue 19 pp 23376—23392

Potentiated lung adenocarcinoma (LUAD) cell growth, migration and invasion by lncRNA DARS-AS1 via miR-188-5p/ KLF12 axis

Overexpression of DARS-AS1 resulted in accelerated LUAD progression. (A) Expression of DARS-AS1 was significantly higher in PC-9 cells transfected with DARS-AS1 ov compared to the NC group (p B) PC-9 cells with DARS-AS1 ov transfection exhibited greater proliferative capability according to the (B) CCK-8 (p C) BrdU (p D) colony formation (p E) Flow cytometry results showed a significantly lower apoptosis rate in the DARS-AS1 ov transfection group (upper right quadrant + lower right quadrant) compared to the NC group (p F) migrating (p G) invading (p

Figure 3. Overexpression of DARS-AS1 resulted in accelerated LUAD progression. (A) Expression of DARS-AS1 was significantly higher in PC-9 cells transfected with DARS-AS1 ov compared to the NC group (p < 0.01). (B) PC-9 cells with DARS-AS1 ov transfection exhibited greater proliferative capability according to the (B) CCK-8 (p < 0.05), (C) BrdU (p < 0.01), and (D) colony formation (p < 0.01) assays compared to the NC group. (E) Flow cytometry results showed a significantly lower apoptosis rate in the DARS-AS1 ov transfection group (upper right quadrant + lower right quadrant) compared to the NC group (p < 0.01). PC-9 with DARS-AS1 ov transfection exhibited a higher number of (F) migrating (p < 0.01) and (G) invading (p < 0.01) cells compared to the NC group.